THE ASSOCIATE EFFECT OF DEPLETED WATER AND MAGNETIC PARTICLES ON Vitis vinifera (L) ORGANOGENESIS

Gallia Butnaru^1*P, Alin Dobrei^1P, Ioan Stefanescu^2P, Gheorghe Titescu^2R,
Alexandra Boleman^1PhD, Diana Costinel^2PhDS

1Banat University of Agricultural Sciences and Veterinary Medicine, Timisoara Romania,
Calea Aradului no. 119, code 300465; Ph. 0040.256.727219; E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it .
²National Institute of Research-Development for Isotopic and Cryogen Technologies-ICSI Ramnicu Valcea, Romania; Uzinei str. No.4, CP7, code 240050; Ph. 0040.250.732744
*Academy of Romanian Scientists full member; P- Professor; R-Senior researcher, PhDS-PhD student

ABSTRACT
 
The effect of depleted water (DDW) associated with Magnetic Nano Particles (MNPs) on organogenesis evolution of the Vitis vinifera (L) was investigated. A total of 10 cuttings of first year shoots belonging to three varieties were put into liquid medium (500 cm3 / jar) in three experimental groups and one control (C) each in four replications. The experiment was one year long being divided in two parts, laboratory and greenhouse conditions respectively. During the laboratory experiment, no nutrients were added in experimental solutions. The number of roots, sprouts and leafs were determined periodically. The capacity to perform organogenesis depended on genotype. Each of them pointed out a particular behaviour.  
In lab conditions the shoot number significantly differs in each variety/liquid composition (P≤0.001). In green house condition on Burgund variety the shoot growth was very fast. At 90 from potting the shoots length varied from 7.36±1.88 to 14.39±4.53 cm in DDW and Negative Control (NC). The number of leafs after 3 months varied from 3.75±0.41 to 7.59±0.45 on Silvania NC and Socodor TW&MNPs respectively. The largest leaves area was 20.32±0.04 cm2 on Burgund variety in DDW conditions. The DDW&MNPs repressed the organogenesis on Silvania and Burgund varieties. The association DDW&MNPs enhanced the leaves area. Generally the single action of DDW enhanced the plantlets traits and improved the chlorophyll content.
The single action of DDW or in association with MNPs has had a favourable influence in organogenesis, growth and synthesis of chlorophyll involved in metabolic activity. The Deuterium amount decreased in leave juice if the plantlets grew in DDW presence.
Key words: deuterium-depleted water (DDW), Magnetic Nano Particles (MNPs), organogenesis, Vitis vinifera (L)