Progress of Cryogenics and Isotopes Separation

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The National Conference with international participation on New Cryogenic and Isotope Technologies for Energy and Environment - EnergEn 2018 is organized by the National Research-Development Institute for Cryogenic and Isotopic Technologies - ICSI Rm. Valcea with the scientific participation of the University of Pitesti and the University of Craiova and will be held at Baile Govora, in 2018.
IDENTIFICATION OF NEW ISOLATES (MICRO-ORGANISMS) FROM VINEYARD EPIPHYTIC MICROFLORA USING MALDI-TOF MASS SPECTROMETRY Print

IDENTIFICATION OF NEW ISOLATES (MICRO-ORGANISMS) FROM VINEYARD EPIPHYTIC MICROFLORA USING MALDI-TOF MASS SPECTROMETRY

Iuliana Diana Bărbulescu1*, Constanţa Mihai2, Mihaela Begea3, Mihai Frîncu2, Liviu Grigorică4, Gabriel Roceanu5, Simona-Ioana Marinescu1, Răzvan Teodorescu2, Radu Tamaian6,7,8*


1Pharmacorp Innovation SRL, Bucharest, Romania; 2University of Agronomic Sciences and Veterinary Medicine Bucharest, Romania; 3University Politehnica of Bucharest, Romania; 4Bevitech SRL, Bucharest, Romania; 5Carl Reh Winery SRL, Bucharest, Romania; 6National Research and Development Institute for Cryogenics and Isotopic Technologies - ICSI Rm. Valcea, Uzinei Street no. 4, P.O. Box Râureni 7, 240050, Râmnicu Vâlcea, Romania; 7University of Bucharest, Faculty of Physics, 3Nano-SAE Research Centre, Bucharest-Măgurele, Romania; 8Biotech Corp SRL, 4th Uzinei Street, Râmnicu Vâlcea, Romania

ABSTRACT

This paper presents the identification of new isolates (micro-organisms) from mature grapes berries & leaves of specific grape varieties and soil from Oprișor area, using MALDI-TOF mass spectrometry. Red grape varieties taken in study were: Cabernet Sauvignon, two Merlot varieties and Shiraz. In the previous study we identified yeast by MALDI-TOF from this wine area. For each variety of red grapes, leaves and soil samples have been also studied. The main micro-organisms from soil were Bacillus sp. from Cabernet area. From Shiraz and Merlot grapes samples the MALDI-TOF score value was 1.26 respectively 1.552, indicating a not reliable identification using MALDI-TOF mass spectrometry. These samples have been also characterized from microscopic and macroscopic point of view. Part of the samples which did not give reliable results by MALDI-TOF mass spectrometry will be the object for the next studies for more precise identification using techniques of molecular taxonomy.

Keywords: MALDI-TOF mass spectrometry, wines, yeast, bacteria.

 

 

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*Corresponding authors: Diana Iuliana Barbulescu, E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it and Radu Tamaian, E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it phone: 0250 732744, fax: 0250 732746