Progress of Cryogenics and Isotopes Separation

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The National Conference with international participation on New Cryogenic and Isotope Technologies for Energy and Environment - EnergEn 2018 is organized by the National Research-Development Institute for Cryogenic and Isotopic Technologies - ICSI Rm. Valcea with the scientific participation of the University of Pitesti and the University of Craiova and will be held at Baile Govora, in 2018.
HPLC DETERMINATION OF OLEANOLIC AND URSOLIC ACID IN APPLES AND APPLE POMACE Print

HPLC DETERMINATION OF OLEANOLIC AND URSOLIC ACID IN APPLES AND APPLE POMACE

 

 

Elisabeta-Irina Geană1, Roxana Elena Ionete1, Alexandru Ciocarlan2,*, Aculina Aricu2, Ala Fulga2, Nicon Ungur2, Marina Podogova3, Diana Nikolaeva3

 

1National Research and Development Institute for Cryogenics and Isotopic Technologies - ICSI Rm. Valcea,

 

Uzinei Street no. 4, P.O. Box Râureni 7, 240050, Râmnicu Vâlcea, Romania; e-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

 

 

ABSTRACT

 

Four different apples cultivars (Semirenko, Golden, Idared and Vagner) and two samples of apple pomace resulted from industrial juice production were subjected to immersion, Soxhlet and supercritical extractions in order to perform full extraction of ursolic (UA) and oleanolic acids (OA) from the mentioned sources. A simple and accurate RP-HPLC method for the determination of UA and OA in extracts obtained by different extraction methods was developed. According to HPLC analyses cuticular extracts from apple peels showed high contents of UA (398.50-541.91 mg/g) and OA (145.72-186.71 mg/g) which are present in a mixture from 2.2:1 to 3.7:1 ratios. The extracts obtained from pomace by Soxhlet extraction are much richer in triterpenic acids with respect to those obtained with subcritical fluid extraction. Both extraction methods proved that apple pomace is a suitable source of bioactive compounds such as ursolic and oleanolic acids.

 

Keywords: HPLC; oleanolic acid; ursolic acid, triterpenes, apples, pomace.


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*Corresponding author: Alexandru Ciocarlan, phone: + 373(22)739790; fax: +373(22)739611;    E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

2Institute of Chemistry, Academy of Sciences of Moldova, Academiei str. 3, MD-2028 Chisinau, Moldova, email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it

3Scientific&Practical Institute of Horticulture, Viticulture and Food Technology, Laboratory of Food Technology, Vieru str. 59, MD-2070, Chisinau, Moldova