Progress of Cryogenics and Isotopes Separation

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The National Conference with international participation on New Cryogenic and Isotope Technologies for Energy and Environment - EnergEn 2018 is organized by the National Research-Development Institute for Cryogenic and Isotopic Technologies - ICSI Rm. Valcea with the scientific participation of the University of Pitesti and the University of Craiova and will be held at Baile Govora, in 2018.
EXPLORING APIS MELLIFERA VENOM COMPOUNDS USING HIGHLY EFFICIENT METHODS Print

EXPLORING APIS MELLIFERA VENOM COMPOUNDS USING HIGHLY EFFICIENT METHODS

 

 

Roxana Elena Ionete, Oana Romina Dinca, Radu Tamaian*, Elisabeta Irina Geana 

  

 

National Institute for Research and Development for Cryogenics and Isotopic Technologies - ICIT Rm. Valcea,

 

Uzinei Street no. 4, P.O. Box Raureni 7, 240050, Ramnicu Valcea, Romania

 

 

ABSTRACT

 

 The aim of this study was to develop an accurate and efficient technique based on combined chromatographic and mass-spectrometry methods for simultaneous determination and characterization of major peptidic fractions from crude honeybee venom (apitoxin). This combined technique may contribute significantly to the standardization and authentication of natural products with peptidic/proteic components. Among bee products, the perfect source of pharmacologically active compounds is represented by the bee venom. The collected venom from bees (Apis mellifera) was analyzed by high performance liquid chromatography (HPLC) and MALDI-TOF mass-spectrometry. The proteic components (melittin, apamin and phospholipase A2) were separated, determined and in consequence a spectra database with proteic fingerprints was created for further use in the authentication procedure. To highlight its applicability, the combined method was validated in order to demonstrate its selectivity, linearity, limit of detection, limit of quantification, accuracy, recovery and stability. Due to its facile use, this combined technique can be easily incorporated into routine operations and used to evaluate quality and authenticity of honeybee venom samples.

 

Keywords: Apis mellifera, apitoxin, HPLC, MALDI-TOF, protein.


*Corresponding author: Radu Tamaian, phone: 0250.732.744; fax: 0250.732.746; E-mail: This e-mail address is being protected from spambots. You need JavaScript enabled to view it